Vernalization Promotes GA-Mediated Bolting Initiation via the Inhibition of ABA and JA Biosynthesis

To understand the relationship between vernalization and endogenous phytohormones on bolting, phytohormone levels transcriptome changes were measured by LC-MS/MS RNA sequencing before (CK), at end of 16 weeks (Vel) 5 days after (Re). A total 32 compounds significantly changed vernalization; especially, content abscisic acid (ABA) jasmonic (JA) was dramatically decreased more than sixteen three times in Vel Re samples. In addition, GA19 accumulated vernalization, while GA53 GA20 decreased. 7471 differentially expressed genes (DEGs) identified response to vernalization. These DEGs enriched with GO terms including stimulus (GO:0050896), hormone (GO:0009725) shoot system development (GO:0048367). KEGG mapping indicated that 16, 13 11 involved gibberellic (GA), ABA JA biosynthesis, suggesting a negative role biosynthesis. Vernalization also repressed expression BvABFs BvMYC2s, implying inhibition signalling. Additionally, plays positive GA signalling, owing down-regulation BvDELLAs. We found GA3-induced bolting could be retarded exogenous methyl jasmonate (MeJA). total, our results suggest can promote GA-induced decreasing BvDELLA repressors signalling eliminating antagonistic effects JA.